Anion exchange resins in phosphate form as versatile carriers for the reactions catalyzed by nucleoside phosphorylases

• Julia N. Artsemyeva,
• Ekaterina A. Remeeva,
• Tatiana N. Buravskaya,
• Irina D. Konstantinova,
• Roman S. Esipov,
• Anatoly I. Miroshnikov,
• Natalia M. Litvinko and
• Igor A. Mikhailopulo

Beilstein J. Org. Chem. 2020, 16, 2607–2622, doi:10.3762/bjoc.16.212

• were tested in the synthesis of nelarabine, kinetin riboside, and cladribine with good to excellent yields (52–93%). Keywords: anion exchange resins; N6-benzyladenosine; cladribine; enzymatic glycosylation; kinetin riboside; nelarabine; α-ᴅ-pentofuranose-1-phosphates; phosphorolysis of nucleosides

• nucleoside. The possibility of using the filtrate obtained in the 1st way as a source of PF-1Pis was tested by the enzymatic synthesis of nelarabinе and kinetin riboside (KR). The choice of these two nucleosides was primarily determined by their biological activities: nelarabine was approved by the Food and

• Supporting Information File 1, SI-1, SI-2 and SI-3). Synthesis of kinetin riboside (KR) The synthesis of KR was performed essentially as described above for nelarabine. The first step of the synthesis, i.e., phosphorolysis of uridine catalyzed by E. coli UP, was realized in water milieu in the presence of